Association Genetics Resistance Gene Enrichment Sequencing (AgRenSeq)

To clone as many disease resistance (R) genes as possible in wild barley, we are utilizing the protocol of AgRenSeq (Arora et al. 2019) on a subset (N=203) of the WBDC. AgRenSeq, as the moniker implies, couples association genetics with RenSeq and can greatly hasten R-gene cloning from plants because it targets not just one gene as in most cloning projects, but potentially a large portion of the NLR complement (i.e. genes with nucleotide binding and leucine rich repeat domains) of a germplasm panel.